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ビデオ・アーカイブ

本領域の事業の一環として,細胞運動のビデオのオンラインライブラリーを作成します.細菌,真核生物,アーキア(古細菌),ウイルス,タンパク質, 合成ポリマー,など様々なものの動きを公開します.それぞれのビデオは,私たちが生物学的に掲載価値があるかどうかを判断,分類し,和文と英文で解説します.

ライブラリー作成のため,皆さまに,(1) 研究者によるご自身の研究対象の投稿,(2) スーパーサイエンスハイスクールや生物部の活動などで顕微鏡をのぞいていて見つけた微生物の投稿,などをお願いします.また,(3) 論文のビデオなどで当ライブラリーにリンクしてほしいもの,(4) 周囲に眠っている古いビデオ教材などでアーカイブ化の価値がありそうなもの,については領域事務局までご一報ください.

ライブラリーのアクセスランキングを下記のリンク先で公開しています。直近の3か月のアクセス数の多いビデオ10本を見ることができます。

また、ビデオ・アーカイブをより手軽に楽しんで頂くために、閲覧用スマートフォンアプリを開発いたしました。
以下からダウンロードできますので、是非ご覧下さい。

ビデオ・アーカイブの収録ビデオの利用に関しては下記へご連絡下さい。

伊藤政博 (masahiro.ito@toyo.jp)
東洋大学生命科学部生命科学科 教授
〒374-0193 群馬県邑楽郡板倉町泉野1-1-1
電話&FAX:0276-82-9202(研究室)、0276-82-9305(5105実験室)

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アクセスランキング

2014.08.28

原核生物
Fluorescent bead movement in the gastric lumen after epithelial damage

Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio, United States of America Professor Marshall H. Montrose

Rheological properties of the in vivo injury site environment has been measured. 105 fluorescent beads (1.0 mm diameter) were added to the gastric luminal superfusate, and then photodamage of gastric surface epithelial cells was induced by two photon laser. Fluorescent beads moved away from gastric tissue after damage, suggesting that the injury creates fluid flow away from the tissue into the lumen

Plos Pathogens

2014.08.28

原核生物
Helicobacter pylori swimming of the wild type strain and straight cell mutant in broth medium

種名:Helicobacter pylori
Molecular and Cellular Biology Graduate Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America Professor Nina R. Salama

Five second video with a frame rate of 0.1 seconds taken at 600×. The straight csd4 mutant is on the left, the wild-type on the right, although the cell morphology appear similar in this magnification. Both strains exhibit similar motility under these conditions.

Plos Pathogens

2014.08.28

原核生物
Helicobacter pylori swimming of the wild-type and straight cell mutant in 0.5% methylcellulose

種名:Helicobacter pylori
Molecular and Cellular Biology Graduate Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America Professor Nina R. Salama

Five second video with a frame rate of 0.1 seconds taken at 600×. The straight csd4 mutant is on the left, the wild-type on the right, although the cell morphology appear similar in this magnification. Both strains exhibit similar motility under these conditions.

Plos Pathogens

2014.08.27

原核生物
“Jerky” motility of Myxococus xanthus gltH mutans

種名:Myxococcus xanthus
Institut de Microbiologie de la Me´diterrane´e (IFR88)–Laboratoire de Chimie Bacte´rienne, CNRS UPR 9043, Marseille, France Tam Mignot

Although mutants deficient for gltA-H and gltK genes retained intact twitching motility on agar plate, single cell motility was completely deficient. These mutants became completely non-motile both at the colony and single cell levels, when pilA was additionally depleted. While, the mutant of gltH pilA showed a jerky motion (small scales displacements) on occasions.

Plos genetics

2014.08.27

原核生物
Localization of GltD-mCherry in differents z-planes in Myxococcus xanthus

種名:Myxococcus xanthus
Institut de Microbiologie de la Me´diterrane´e (IFR88)–Laboratoire de Chimie Bacte´rienne, CNRS UPR 9043, Marseille, France Professor Tam Mignot

It has been demonstrated that the aglRQS encodes a proton-motive force-driven channel that produces motility traction at FACs (Focal Adhesion Complexes). Glt proteins may be functionally related to AaglRQSs. The localization pattern of GltD-, GltF-mCherry fluorescence in live cells was around the cell periphery in good agreement with AaglRQSs.

Plos Genetics

2014.08.27

原核生物
Sporulation of Myxococcus cells in the microfluidic chamber

種名:Myxococcus xanthus
Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

Myxococcus cells couldn’t complete the sporulation process when they spotted directly on an agar pad. To solve this problem under the live microscopy and elucidate how the Agl-Nfs machinery drives spore coat assembly, a microfluidic chamber assay has been developed. In this way, cells are immobilized and sporulate in liquid directly on the microscope stage. Under these conditions, viable spores were obtained approximately 250–300 min after glycerol addition.

Plos Biology

2014.08.27

原核生物
3D reconstruction of a spore expressed BtkA-sfGFP and stained by FM4-64

種名:Myxococcus xhantus
Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

Myxococcus synthes spore coat by a group of gene (exoA–I). The function of this genes is encoding a capsular polysaccharide, and Wza-type export apparatus. The movie is showing the localization of structural components of the export apparatus. As an exception of proteobacteria, in Myxococcus the Wzc transmembrane domain and the kinase Wzc domain are carried by two distinct polypeptides named ExoC and ExoD/BtkA, respectively. BtkA is essential for Myxococcus sporulation, suggesting that BtkA can be used to localize Exo export sites. Z-sections of 4-h-old spores and 3D reconstructions further revealed that BtkA-sfGFP foci form at discrete sites around the spore periphery. The Z-stack recording was obtained on a 4-h-old sporulating cell.

Plos Biology

2014.08.27

モデル(解説を含む)
Movement of an unpolarized cell in changing gradients

Department of Electrical and Computer Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America Professor Pablo A. Iglesias

Simulation of the LEGI-BEN module under changing 19% gradients. The initial 19% gradient, which points to the top was applied at 180 s. At 500 s, it was switched to point towards the bottom

Plos Computational Biology

2014.08.27

モデル(解説を含む)
Response of unpolarized cell to simultaneous gradients

Department of Electrical and Computer Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America Professor Pablo A. Iglesias

Competing 19% gradients were applied to the cell. This simulation uses the LEGI and EN modules. The red line marks the track of the cell centroid

Plos Computational Biology

2014.08.27

モデル(解説を含む)
Simulation of cells lacking adaptation in higher midpoint concentration

Department of Electrical and Computer Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America Professor Pablo A. Iglesias

This simulation shows the response of the cell to a 19% gradient pointing to the right. The inhibitor level of the LEGI mechanism has been set constant, so the cell cannot adapt or adjust sensitivity. The midpoint chemoattractant concentration has been increased.

Plos Computational Biology

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