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ビデオ・アーカイブ

本領域の事業の一環として,細胞運動のビデオのオンラインライブラリーを作成します.細菌,真核生物,アーキア(古細菌),ウイルス,タンパク質, 合成ポリマー,など様々なものの動きを公開します.それぞれのビデオは,私たちが生物学的に掲載価値があるかどうかを判断,分類し,和文と英文で解説します.

ライブラリー作成のため,皆さまに,(1) 研究者によるご自身の研究対象の投稿,(2) スーパーサイエンスハイスクールや生物部の活動などで顕微鏡をのぞいていて見つけた微生物の投稿,などをお願いします.また,(3) 論文のビデオなどで当ライブラリーにリンクしてほしいもの,(4) 周囲に眠っている古いビデオ教材などでアーカイブ化の価値がありそうなもの,については領域事務局までご一報ください.

ライブラリーのアクセスランキングを下記のリンク先で公開しています。直近の3か月のアクセス数の多いビデオ10本を見ることができます。

また、ビデオ・アーカイブをより手軽に楽しんで頂くために、閲覧用スマートフォンアプリを開発いたしました。
以下からダウンロードできますので、是非ご覧下さい。

ビデオ・アーカイブの収録ビデオの利用に関しては下記へご連絡下さい。

伊藤政博 (masahiro.ito@toyo.jp)
東洋大学生命科学部生命科学科 教授
〒374-0193 群馬県邑楽郡板倉町泉野1-1-1
電話&FAX:0276-82-9202(研究室)、0276-82-9305(5105実験室)

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アクセスランキング

2014.08.29

真核生物
High speed recording of a tumbling walker of Trypanosoma brucei brucei

種名:Trypanosoma brucei brucei
Max-Planck-Institute for Dynamics and Self-Organization, Go¨ ttingen, Germany, and Department of Chemistry, University Professor Thomas Pfohl
of Basel, Basel, Switzerland 

Recorded at 1000 frames per second and at 60x magnification

Plos Computational Biology

2014.08.29

分子・タンパク質
高速AFMで撮影されたミオシンVの一方向歩行運動

金沢大学理工研究域数物科学、バイオAFM先端研究センター 安藤敏夫、内橋貴之、古寺哲幸

ミオシンVは2つの足を持ったモータータンパク質で、細胞の中では荷物運びを行っていることが分かっています。尾部を取り除いたミオシンV(ミオシンV-HMM)のアクチンフィラメントに沿って一方向に歩行運動する様子を高速AFMで直接観察しました。溶液中には1µMの濃度のATP(ミオシンVのエネルギー源)が入っています。撮影の条件は、1秒間に7フレームの走査速度、走査範囲は130 × 65 nm2 (80 × 40 ピクセル)です。歩行運動中のミオシンVはたまに足踏み運動していることが分かりました。後ろ足の足踏み運動のときに赤い三角のマーク、前足の足踏み運動の時に水色の三角のマークが現れます。

Annual Review of Biophysics 42: 393-414 (2013)
Nature 468: 72-76 (2010)

2014.08.28

原核生物
Fluorescent labeled Helicobacter pylori motility

種名:Helicobacter pylori
Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio, United States of America Professor Marshall H. Montrose

The motility of wild-type H. pylori Strain SS1 was observed by tracking fluorescently labeled cells using a fast-scan confocal microscope. The cells swam in straight or arced lines at mean velocity of 25.5±0.7 µm/sec, with stopping at the frequency of 0.13±0.05 per s, and slowed at pH 5. Fluorescently labeled H. pylori was loaded into an uncoated μ-Slide chamber (ibidi) and monitored on the inverted fast scan confocal microscope (Zeiss LSM 7 LIVE), with a scan speed at 100 ms/frame.

Plos Pathogens

2014.08.28

原核生物
Fluorescent bead movement in the gastric lumen after epithelial damage

Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio, United States of America Professor Marshall H. Montrose

Rheological properties of the in vivo injury site environment has been measured. 105 fluorescent beads (1.0 mm diameter) were added to the gastric luminal superfusate, and then photodamage of gastric surface epithelial cells was induced by two photon laser. Fluorescent beads moved away from gastric tissue after damage, suggesting that the injury creates fluid flow away from the tissue into the lumen

Plos Pathogens

2014.08.28

原核生物
Helicobacter pylori swimming of the wild type strain and straight cell mutant in broth medium

種名:Helicobacter pylori
Molecular and Cellular Biology Graduate Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America Professor Nina R. Salama

Five second video with a frame rate of 0.1 seconds taken at 600×. The straight csd4 mutant is on the left, the wild-type on the right, although the cell morphology appear similar in this magnification. Both strains exhibit similar motility under these conditions.

Plos Pathogens

2014.08.28

原核生物
Helicobacter pylori swimming of the wild-type and straight cell mutant in 0.5% methylcellulose

種名:Helicobacter pylori
Molecular and Cellular Biology Graduate Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America Professor Nina R. Salama

Five second video with a frame rate of 0.1 seconds taken at 600×. The straight csd4 mutant is on the left, the wild-type on the right, although the cell morphology appear similar in this magnification. Both strains exhibit similar motility under these conditions.

Plos Pathogens

2014.08.27

原核生物
“Jerky” motility of Myxococus xanthus gltH mutans

種名:Myxococcus xanthus
Institut de Microbiologie de la Me´diterrane´e (IFR88)–Laboratoire de Chimie Bacte´rienne, CNRS UPR 9043, Marseille, France Tam Mignot

Although mutants deficient for gltA-H and gltK genes retained intact twitching motility on agar plate, single cell motility was completely deficient. These mutants became completely non-motile both at the colony and single cell levels, when pilA was additionally depleted. While, the mutant of gltH pilA showed a jerky motion (small scales displacements) on occasions.

Plos genetics

2014.08.27

原核生物
Localization of GltD-mCherry in differents z-planes in Myxococcus xanthus

種名:Myxococcus xanthus
Institut de Microbiologie de la Me´diterrane´e (IFR88)–Laboratoire de Chimie Bacte´rienne, CNRS UPR 9043, Marseille, France Professor Tam Mignot

It has been demonstrated that the aglRQS encodes a proton-motive force-driven channel that produces motility traction at FACs (Focal Adhesion Complexes). Glt proteins may be functionally related to AaglRQSs. The localization pattern of GltD-, GltF-mCherry fluorescence in live cells was around the cell periphery in good agreement with AaglRQSs.

Plos Genetics

2014.08.27

原核生物
Sporulation of Myxococcus cells in the microfluidic chamber

種名:Myxococcus xanthus
Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

Myxococcus cells couldn’t complete the sporulation process when they spotted directly on an agar pad. To solve this problem under the live microscopy and elucidate how the Agl-Nfs machinery drives spore coat assembly, a microfluidic chamber assay has been developed. In this way, cells are immobilized and sporulate in liquid directly on the microscope stage. Under these conditions, viable spores were obtained approximately 250–300 min after glycerol addition.

Plos Biology

2014.08.27

原核生物
3D reconstruction of a spore expressed BtkA-sfGFP and stained by FM4-64

種名:Myxococcus xhantus
Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

Myxococcus synthes spore coat by a group of gene (exoA–I). The function of this genes is encoding a capsular polysaccharide, and Wza-type export apparatus. The movie is showing the localization of structural components of the export apparatus. As an exception of proteobacteria, in Myxococcus the Wzc transmembrane domain and the kinase Wzc domain are carried by two distinct polypeptides named ExoC and ExoD/BtkA, respectively. BtkA is essential for Myxococcus sporulation, suggesting that BtkA can be used to localize Exo export sites. Z-sections of 4-h-old spores and 3D reconstructions further revealed that BtkA-sfGFP foci form at discrete sites around the spore periphery. The Z-stack recording was obtained on a 4-h-old sporulating cell.

Plos Biology

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