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ビデオ・アーカイブ

本領域の事業の一環として,細胞運動のビデオのオンラインライブラリーを作成します.細菌,真核生物,アーキア(古細菌),ウイルス,タンパク質, 合成ポリマー,など様々なものの動きを公開します.それぞれのビデオは,私たちが生物学的に掲載価値があるかどうかを判断,分類し,和文と英文で解説します.

ライブラリー作成のため,皆さまに,(1) 研究者によるご自身の研究対象の投稿,(2) スーパーサイエンスハイスクールや生物部の活動などで顕微鏡をのぞいていて見つけた微生物の投稿,などをお願いします.また,(3) 論文のビデオなどで当ライブラリーにリンクしてほしいもの,(4) 周囲に眠っている古いビデオ教材などでアーカイブ化の価値がありそうなもの,については領域事務局までご一報ください.

ライブラリーのアクセスランキングを下記のリンク先で公開しています。直近の3か月のアクセス数の多いビデオ10本を見ることができます。

また、ビデオ・アーカイブをより手軽に楽しんで頂くために、閲覧用スマートフォンアプリを開発いたしました。
以下からダウンロードできますので、是非ご覧下さい。

ビデオ・アーカイブの収録ビデオの利用に関しては下記へご連絡下さい。

伊藤政博 (masahiro.ito@toyo.jp)
東洋大学生命科学部生命科学科 教授
〒374-0193 群馬県邑楽郡板倉町泉野1-1-1
電話&FAX:0276-82-9202(研究室)、0276-82-9305(5105実験室)

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アクセスランキング

2014.09.19

原核生物
Microscopic observation of tumbling Lactobacillus ruminis ATCC25644 cells in MRS broth

種名:Lactobacillus ruminis
Department of Microbiology, University College Cork, Cork, Ireland, and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland Professor Paul W. O’Toole

Video was recorded with a USB eyepiece camera attached to a phase-contrast microscope.

Plos One

2014.09.19

原核生物
Vibrio anguillarum wilde type evades phagocytosis by rainbow trout skin epithelial cells

Department of Molecular Biology, Umea° Centre for Microbial Research, Umea° University, Umea°, Sweden, and Southern Research Institute, Birmingham, Alabama, United Professor Debra L. Milton
States of America 

Skin epithelial cells were isolated from rainbow trout and infected with 105 bacteria ml−1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy.

Plos One

2014.09.19

原核生物
Vibrio anguillarum Δwzt mutant, is phagocytized by rainbow trout skin epithelial cells

Department of Molecular Biology, Umea° Centre for Microbial Research, Umea° University, Umea°, Sweden, and Southern Research Institute, Birmingham, Alabama, United Professor Debra L. Milton
States of America 

Δwzt mutant is defective for O-antigen transport. Skin epithelial cells were isolated from rainbow trout and infected with 105 bacteria ml−1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy.

Plos One

2014.09.19

原核生物
Vibrio anguillarum Δwzt mutant carrying the wild-type wzt gene regains

Department of Molecular Biology, Umea° Centre for Microbial Research, Umea° University, Umea°, Sweden, and Southern Research Institute, Birmingham, Alabama, United Professor Debra L. Milton
States of America 

This video shows the ability of completed mutant to evade phagocytosis by the rainbow trout skin epithelial cells. Skin epithelial cells were isolated from rainbow trout and infected with 105 bacteria ml−1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy. This video is representative of epithelial cell interactions also associated with the Δwzm and ΔwbhA mutants carrying the respective complementing wild-type gene. All three complemented mutant strains regained the ability to evade phagocytosis.

Plos One

2014.09.19

原核生物
Mannose blocks phagocytosis of the Vibrio anguillarum Δwzt mutant

Department of Molecular Biology, Umea° Centre for Microbial Research, Umea° University, Umea°, Sweden, and Southern Research Institute, Birmingham, Alabama, United Professor Debra L. Milton
States of America 

Rainbow trout skin epithelial cells were isolated and incubated with 1 mM mannose prior to infection with 105 bacteria ml−1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy. This video suggests that the epithelial cells utilize a mannose-binding receptor to bind sugar residues on the surface of V. anguillarum strains lacking the O-antigen. The O-antigen may be predicted to mask these sugar residues on the bacterial surface blocking phagocytosis.

Plos One

2014.09.18

原核生物
Salmonella Typhimurium swimming along the surface of HeLa cells (Small version)

Institute of Microbiology, D-BIOL, ETH Zu¨ rich, Zurich, Switzerland Professor Wolf-Dietrich Hardt

HeLa cells were infected with S.TmΔ4 at an m.o.i. of 0.5and a DIC movie was acquired using a 63× objective at 23 frames per second and is shown in real time. S. Typhimurium move in and out of focus following the cellular surface before stopping at a mitotic cell.

Plos Pathogens

2014.09.18

原核生物
Salmonella Typhimurium swimming along the surface of HeLa cells (Large version)

Institute of Microbiology, D-BIOL, ETH Zu¨ rich, Zurich, Switzerland, Professor Wolf-Dietrich Hardt

HeLa cells were infected with S.TmΔ4 at an m.o.i. of 0.5and a DIC movie was acquired using a 63× objective at 23 frames per second and is shown in real time. S. Typhimurium move in and out of focus following the cellular surface before stopping at a mitotic cell.

Plos Pathogens

2014.09.18

原核生物
Salmonella typhimurium NSS on a glass surface with glass bead obstacles

Institute of Microbiology, D-BIOL, ETH Zu¨ rich, Zurich, Switzerland, Professor Wolf-Dietrich Hardt

Gelatin coated glass beads (500 µm diameter) were placed onto a glass-bottom dish filled with HBSS buffer and the swimming behavior of S.Tmwt(mCherry) was recorded (20 frames per second; 300 frames) by confocal fluorescence microscopy using a 100× objective.

Plos Pathogens

2014.09.18

原核生物
Stopping of Salmonella typhimurium at cellular ruffles

Institute of Microbiology, D-BIOL, ETH Zu¨ rich, Zurich, Switzerland Professor Wolf-Dietrich Hardt

HeLa cells were infected with a 1:1 mixture of S.TmΔ4(pGFP) and S.TmSopE(pM2112, constitutive rfp-plasmid) at an m.o.i. of 5,6 minutes before movie acquisition. A 2.5 minute DIC movie was acquired using a 63× objective and 2-fold optovar, at 23 frames per second and is shown in real time. Images in the fluorescence channel showed that both S.TmΔ4 (non-invasive mutant) and S.TmSopE (invasive mutant) were associated with the membrane ruffle.

Plos Pathogens

2014.09.17

モデル(解説を含む)
Polymerization of actin

Department of Chemistry and Biochemistry at UCSD Professor McCammon

This polymerization of actin both within the cell and in vitro has been studied experimentally for many years. One of the most interesting facts about actin polymerization is that the two ends of the filament do not grow at the same rate, but the barbed polymerizes 5-20 times faster than the pointed end. The basis for this asymmetry has never truly been understood. With advances in computational power in the past years, it has become possible to simulate the interaction of larger biomolecules, the polymerization of actin being one such system. Using Brownian dynamics simulations, the binding of a monomer to the end of a filament was simulated over a range of ionic strengths. The animation depicts two `fictional' trajectories intended to illustrate the basic concepts behind the simulations. For each trajectory, the monomer was started with a random orientation on a sphere surrounding the filament. By keeping track of the number of successful binding events at each end of the filament, a rate constant for binding can be calculated. This study showed that electrostatic interactions in fact lead to an asymmetry in polymerization rates between the two ends.

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