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ビデオ・アーカイブ

本領域の事業の一環として,細胞運動のビデオのオンラインライブラリーを作成します.細菌,真核生物,アーキア(古細菌),ウイルス,タンパク質, 合成ポリマー,など様々なものの動きを公開します.それぞれのビデオは,私たちが生物学的に掲載価値があるかどうかを判断,分類し,和文と英文で解説します.

ライブラリー作成のため,皆さまに,(1) 研究者によるご自身の研究対象の投稿,(2) スーパーサイエンスハイスクールや生物部の活動などで顕微鏡をのぞいていて見つけた微生物の投稿,などをお願いします.また,(3) 論文のビデオなどで当ライブラリーにリンクしてほしいもの,(4) 周囲に眠っている古いビデオ教材などでアーカイブ化の価値がありそうなもの,については領域事務局までご一報ください.

ライブラリーのアクセスランキングを下記のリンク先で公開しています。直近の3か月のアクセス数の多いビデオ10本を見ることができます。

また、ビデオ・アーカイブをより手軽に楽しんで頂くために、閲覧用スマートフォンアプリを開発いたしました。
以下からダウンロードできますので、是非ご覧下さい。

ビデオ・アーカイブの収録ビデオの利用に関しては下記へご連絡下さい。

伊藤政博 (masahiro.ito@toyo.jp)
東洋大学生命科学部生命科学科 教授
〒374-0193 群馬県邑楽郡板倉町泉野1-1-1
電話&FAX:0276-82-9202(研究室)、0276-82-9305(5105実験室)

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アクセスランキング

2014.09.18

原核生物
Salmonella typhimurium NSS on a glass surface with glass bead obstacles

Institute of Microbiology, D-BIOL, ETH Zu¨ rich, Zurich, Switzerland, Professor Wolf-Dietrich Hardt

Gelatin coated glass beads (500 µm diameter) were placed onto a glass-bottom dish filled with HBSS buffer and the swimming behavior of S.Tmwt(mCherry) was recorded (20 frames per second; 300 frames) by confocal fluorescence microscopy using a 100× objective.

Plos Pathogens

2014.09.18

原核生物
Stopping of Salmonella typhimurium at cellular ruffles

Institute of Microbiology, D-BIOL, ETH Zu¨ rich, Zurich, Switzerland Professor Wolf-Dietrich Hardt

HeLa cells were infected with a 1:1 mixture of S.TmΔ4(pGFP) and S.TmSopE(pM2112, constitutive rfp-plasmid) at an m.o.i. of 5,6 minutes before movie acquisition. A 2.5 minute DIC movie was acquired using a 63× objective and 2-fold optovar, at 23 frames per second and is shown in real time. Images in the fluorescence channel showed that both S.TmΔ4 (non-invasive mutant) and S.TmSopE (invasive mutant) were associated with the membrane ruffle.

Plos Pathogens

2014.09.17

モデル(解説を含む)
Polymerization of actin

Department of Chemistry and Biochemistry at UCSD Professor McCammon

This polymerization of actin both within the cell and in vitro has been studied experimentally for many years. One of the most interesting facts about actin polymerization is that the two ends of the filament do not grow at the same rate, but the barbed polymerizes 5-20 times faster than the pointed end. The basis for this asymmetry has never truly been understood. With advances in computational power in the past years, it has become possible to simulate the interaction of larger biomolecules, the polymerization of actin being one such system. Using Brownian dynamics simulations, the binding of a monomer to the end of a filament was simulated over a range of ionic strengths. The animation depicts two `fictional' trajectories intended to illustrate the basic concepts behind the simulations. For each trajectory, the monomer was started with a random orientation on a sphere surrounding the filament. By keeping track of the number of successful binding events at each end of the filament, a rate constant for binding can be calculated. This study showed that electrostatic interactions in fact lead to an asymmetry in polymerization rates between the two ends.

2014.09.17

原核生物
Myxococcus motility on agar

Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

Gram-negative bacterium Myxococcus xanthus used as a model system to address these fundamental questions. Specifically, using a multidisciplinary approach from the realms of genetics, biochemistry, cell biology, bioinformatics and quantitative physics to study the motility mechanism and its regulation in response to environmental cues, both at the single and multicellular levels. In the longer term, our goal is to build a high-resolution model of a bacterial multicellular life cycle integrating knowledge from experiments and computational simulations.

2014.09.17

モデル(解説を含む)
Myxococcus move on surfaces?

Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

We are interested in determining the mechanism of the so-called Myxococcus (A)-motility, a process where the bacterial cell moves smoothly along its long axis in absence of obvious extracellular organelles. Combining genetics, cell biology and physics, we have recently identified the motility machinery for the first time. Work from ours and other laboratories have led to a working model, proposing that ventral assembly of the motility complex promotes movement. However, how the machinery propels the cells is still obscure. How are traction forces produced? Is there a connection with the cytoskeleton? How is the directionality of the system dictated? Are some of the questions that we are trying to address. To do this, we are combining techniques from the realms of genetics, cell biology and physics. For more information, see Mignot et al. (2007), Sun, Wartel et al. (2011), Luciano, Agrebi et al. (2011).

2014.09.17

原核生物
How do Myxococcus cells direct their motility?

Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

Myxococcus cells can change their direction of movement by a process called a reversal where the poles exchange roles, allowing the bacteria to rapidly move in the opposite direction. Such rapid directional changes result from pole-to-pole switching of a central Ras-like small G-protein, MglA. Genetic control of these switches is at the heart of the Myxococcus multicellular lifestyle, swarming, prdation and fruiting body formation. How is MglA localization controlled dynamically? What signalling pathways control MglA localization? How is MglA regulating motility? How are those regulations affecting cell-cell cooperation in groups? For more information, see Mignot et al. (2005), Mauriello, Mouhamar et al. (2010), Zhang et al. (2010).

2014.09.17

原核生物
Chemoreceptor alignment following Myxococcus cell contact

Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot

environment? Multicellular cooperation arises from cell-cell signalling and environment sensing and chemotaxis. The localization of a Myxococcus xanthus cytoplasmic receptor (Methyl-accepting Chemotaxis Protein, MCP) changes dynamically in response to cell contacts. This observation suggests that Myxococcus cells possess a “sense of touch” allowing cells to respond to the external environment by modulating coordinated cell movement. Myxococcus has a total of 21 MCP encoding genes. Some of them have been characterized genetically and biochemically. Similar to enteric bacteria, these receptors might be cross-linked with each other and form complex arrays ultimately generating sensory machineries. We are trying to characterize these sensory machineries and understand the link between the localization of bacterial chemoreceptors in cells and their function during chemotaxis

2014.09.17

原核生物
Gliding motility of Cellulophaga lytica observed at colony edges by phase contrast microscopy

UMR 7266 CNRS Littoral Environnement et Socie´te´ s, University of La Rochelle, La Rochelle, France Professor Eric Rosenfeld

The bacterium was grown on Cytophaga agar medium and movements were recorded during 1 minute (one photo every three seconds). Gliding reverse movements were visible. Motility was reduced in the central parts of the colony.

Plos One

2014.09.17

原核生物
Gliding motility of Cellulophaga lytica observed at colony extreme edges by phase contrast microscopy

UMR 7266 CNRS Littoral Environnement et Socie´te´ s, University of La Rochelle, La Rochelle, France Professor Eric Rosenfeld

The bacterium was grown on Cytophaga agar medium and movements were recorded during 1 minute (one photo every three seconds). A collective motion and the organization of cells into larger moving clusters were observed

Plos One

2014.09.17

原核生物
Dynamic formation of an iridescent glitter on the edges of a Cellulophaga lytica DSM 2040 colony after 24 h incubation on Cytophaga agar medium

UMR 7266 CNRS Littoral Environnement et Socie´te´ s, University of La Rochelle, La Rochelle, France Professor Eric Rosenfeld

The phenomenon was observed with a total duration of 50 minutes (one photo every minute). On the edges, spreading movements were well visible. Several layers seemed needed to observe the red iridescence from the glitter.

Plos One

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