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アクセスランキング

2014.10.25

真核生物
Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts (No. 2)

Department of Molecular & Cell Biology, University of California–Berkeley, Berkeley, California, United States of America Professor Rebecca Heald

Behavior of a monopolar RCC1 bead spindle. Fluorescence time-lapse movie of Xenopus egg extract spindle assembly reaction containing rhodamine labeled tubulin is shown over a time interval of 21 min. Note that the microtubules appear to push the bead, which is trailed by microtubules.

Plos Biology

2014.10.25

真核生物
Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts (No. 3)

Department of Molecular & Cell Biology, University of California–Berkeley, Berkeley, California, United States of America Professor Rebecca Heald

The effect of coupling kinesin 1 motor domain together with RCC1 to the bead. Two side-by-side fluorescence time-lapse movies of Xenopus egg extract containing rhodamine labeled tubulin and beads coated with RCC1 plus kinesin-1 motor domain, shown over the same time interval of 37 min.

Plos Biology

2014.10.25

真核生物
Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts (No. 4)

Department of Molecular & Cell Biology, University of California–Berkeley, Berkeley, California, United States of America Professor Rebecca Heald

The effect of coupling kinesin 1 motor domain mutant R203K together with RCC1 to the bead. Two side-by-side fluorescence time-lapse movies of egg extract spindle assembly containing rhodamine labeled tubulin and a bead coated either with RCC1 (left) or with RCC1 plus kinesin-1 motor domain ATPase mutant R203K, shown over the same time interval of 49 min.

Plos Biology

2014.10.25

真核生物
Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts (No. 5)

Department of Molecular & Cell Biology, University of California–Berkeley, Berkeley, California, United States of America Professor Rebecca Heald

The effect of coupling EB1 together with RCC1 to the bead. Fluorescence time-lapse movie of egg extract spindle assembly reaction containing rhodamine labeled tubulin and a bead coated with RCC1 plus EB1, shown over a time interval of 40 min. EB1 does not diminish bead oscillation.

Plos Biology

2014.10.25

その他
Microtubule (MT) repolymerization in CHO cells

Department of Molecular Genetics, VIB and University of Antwerp, Antwerpen, Belgium,  Professor Sophie Janssens

CHO HSPB1− (top) and CHO HSPB1+ (bottom) were transfected with EB1-GFP, treated with nocodazole and washed while imaging with spinning disk confocal microscopy. Image z-stacks comprising the complete volume of the cells were acquired every 5 sec. The movie shows the maximum intensity projections of all slices in the z-stack with inverted grey scale. Images on the right are shown with overlaid EB1-GFP tracks. Note much more non-centrosomal and centrosomal MTs were formed in HSPB1+.

Plos One

2014.10.25

分子・タンパク質
Non-centrosomal microtubules (MTs) traced by EB1-GFP in steady state cells

Department of Molecular Genetics, VIB and University of Antwerp, Antwerpen, Belgium, Professor Sophie Janssens

CHO HSPB1− (top) and CHO HSPB1+ (bottom) were transfected with EB1-GFP and imaged with spinning disk confocal microscopy. Image z-stacks comprising the centrosomal and Golgi region were acquired every 3 sec. The images on the left show the maximum intensity projections of all slices in the z-stack with inverted grey scale. Images on the right show these as cumulative maximum intensity projections over the elapsed time. Note the large number of non-centrosomal MTs (Microtubules) formed in CHO-HSPB1+ cells.

Plos One

2014.10.23

モデル(解説を含む)
Part 1: GTP-binding Proteins as Molecular Switches

Max-Planck Institute Professor Alfred Wittinghofer

When a growth factor binds to the plasma membrane of a quiescent cell, an intracellular signaling pathway is activated telling the cell to begin growing. A key molecule in this signaling pathway is the GTP-binding protein, or G-protein, Ras. Ras can act as an on-off switch telling the cell to grow or not. In its inactive form, Ras is bound to GDP while in its active form it is bound to GTP. This exchange of nucleotides is catalysed by guanine nucleotide-exchange-factors (GEFs). The return to the inactive state occurs through the GTPase reaction, which is accelerated by GTPase-activating proteins (GAPs). In Part 1 of his talk, Dr. Wittinghofer explains how solving the three-dimensional structure of Ras, and other G-proteins, allowed him to understand the conserved mechanism by which G-proteins can act as switches. The structure also identified domains unique to each G-protein that provide the specificity for downstream signals.

2014.10.23

モデル(解説を含む)
Part 2: GTPase Reactions and Diseases

Max-Planck Institute Professor Alfred Wittinghofer

In the second part of Dr. Wittinghofer's talk he explains the link between GTPases and disease. Ras is both a key molecule in regulating normal cell growth and an oncogene in unregulated cancer cell growth. Mutations in Ras that prevent the hydrolysis of GTP to GDP lock Ras into an active state rendering it independent of upstream growth factor signals. Biophysical studies from Wittinghofer's lab solved the multiple steps in the hydrolysis of GTP to GDP and explained why particular mutations in either Ras or Ras-GAPs cause unregulated activation of Ras and tumor formation. Examples of other G-proteins that are unable to hydrolyse GTP and result in different diseases such as Retinitis Pigmentosa, are also presented.

ibiology

2014.10.17

原核生物
Live E. coli cells expressing mMaple-CheW

種名:E. coli
Department of Chemistry, University of Alberta, Edmonton, Alberta, Professor Robert E. Campbell
Canada 

3D animation of a structured illumination microscopy (SIM) reconstructed for many live E. coli cells expressing mMaple, an engineered photoconvertible fluorescent protein (pcFP) variant -CheW (top left channel) stained with FM4-64 (top right channel). The channels have been combined in the bottom right panel. Scale bar is 1 µm.

Plos One

2014.10.17

原核生物
Live E. coli expressing GFP-CheW

種名:E. coli
Department of Chemistry, University of Alberta, Edmonton, Alberta, Professor Robert E. Campbell
Canada, 

3D animation of a structured illumination microscopy (SIM) reconstructed for a live E. coli expressing GFP-CheW. The cellular membrane was stained with FM4-64. Scale bar is 500 nm.

Plos One

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